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应用同源PCR技术,从被一种球状病毒感染的患病大菱鲆(Scophthalmus maximus)脾脏和肾脏组织中扩增出了一段长度为620 bp的DNA片断。序列测定和Blast分析表明,该DNA片断与鱼类虹彩病毒主要衣壳蛋白(MCP)C末端编码区的DNA序列高度相似,由此证实感染养殖大菱鲆的这种球状病毒为一种鱼类虹彩病毒,暂命名为大菱鲆红体病虹彩病毒(TRBIV)。多序列比对和分析发现,TRBIV MCP C末端的205个氨基酸序列与GenBank中20种虹彩病毒相应序列的相似性分别为99.47%(韩国大菱鲆虹彩病毒)、97%~98%(待指定病毒属的7种病毒),以及50%以下(蛙病毒属、淋巴囊肿病毒属、虹彩病毒属的12种病毒),由此绘制出了包含TRBIV在内的21种虹彩病毒的系统发育树。研究结果表明,感染中国养殖大菱鲆的TRBIV属于虹彩病毒科待指定病毒属,位于该属ISKNV亚群和RSIV亚群之间,是该病毒属的一个新成员。
Using homologous PCR, a DNA fragment of 620 bp in length was amplified from the spleens and kidneys of scophthalmus maximus infected with a type of coronavirus. Sequence analysis and Blast analysis showed that the DNA fragment was highly similar to the DNA sequence of the coding region of the major capsid protein (MCP) of the fish iridescent virus, thereby confirming that the baculovirus infected with cultured turbot was a fish Iridovirus, tentatively named Turbot’s red tick disease iridescent virus (TRBIV). Multiple sequence alignment and analysis revealed that the similarity of the 205 amino acid sequences at the C terminus of TRBIV to the corresponding sequences of the 20 iridescent viruses in GenBank was 99.47% (97% -98%, respectively) (to be assigned Seven viruses of the genus Viruses) and less than 50% (12 viruses of the genera Frogvirus, Lymphocystivirus, and Iridovirus), thereby drawing a phylogenetic tree of 21 iridescent viruses including TRBIV. The results showed that TRBIV infected Chinese cultured turbot is a member of the family of iridescent virus to be identified and located between ISKNV subgroup and RSIV subgroup, which is a new member of this genus.