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对太行菊属(Opisthopappus)的太行菊(O.taihangensis)和长裂太行菊(O.longilobus)13个种群的核糖体DNA ITS进行测序,分析不同种、不同种群间的ITS序列差异。结果表明:排序后的ITS序列总长度为682 bp,含有15个简约信息位点;根据ITS序列差异共确定出18种单倍型,太行菊和长裂太行菊种群均表现出高的单倍型多样性和核苷酸多样性;两个种均有独有的单倍型,又有共有单倍型;聚类分析表明18种单倍型形成明显两支,Hn1和Hn8分别位于两支的中心为祖先单倍型。ITS序列将太行菊和长裂太行菊13个种群聚类成一个单源支系,但长裂太行菊中两个种群(林虑山LLS和石板岩SBY)与太行菊种群聚为一支,显示出两种之间存在着基因交流或杂交。在进化过程中,长裂太行菊可能经历了长距离侵殖,太行菊在太行山隆升之前经历了种群的扩张,随着太行山的隆升逐渐形成现今的分布格局。
The ribosomal ITS of 13 populations of O.taihangensis and O. longilobus from Opisthopappus was sequenced, and ITS sequences differences among different species and populations were analyzed. The results showed that the total length of sequenced ITS sequences was 682 bp with 15 parsimony informative sites. According to the differences of ITS sequences, a total of 18 haplotypes were identified. Type diversity and nucleotide diversity. Both species had unique haplotypes and common haplotypes. Cluster analysis showed that two haplogroups formed 18 distinct haplotypes, Hn1 and Hn8 were located in two The center is the ancestral haplotype. ITS sequences clustered 13 populations of Taihang chrysanthemum and Long-tailed Taihang chrysanthemum into a single source. However, two populations (LLS and SBY) It shows that there is gene communication or hybridization between the two species. In the process of evolution, the long-tailed Tai chrysanthemum may experience long-term colonization. The Taihang chrysanthemum has undergone population expansion before the uplift of the Taihang Mountains. With the uplift of the Taihang Mountains, the current distribution pattern is gradually formed.