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为连续观察药物对细胞代谢的影响，用含紫杉醇的灌注液对人口腔上皮癌ＫＢ细胞和人结肠癌ＨＣＴ８细胞在灌注条件下进行了细胞３１Ｐ谱测定，从两种细胞在未给药、给药以及停药后的灌注条件下获得的代谢图谱中发现，紫杉醇使ＫＢ细胞和ＨＣＴ８细胞的ＡＴＰ代谢水平均有升高，但ＫＢ细胞的ＡＴＰ升高更为明显；同样剂量的长春新碱使ＫＢ细胞代谢中的ＡＴＰ峰的改变不明显，推测可能与它们的作用机制不同有关。研究结果表明，灌注细胞３１Ｐ谱测试法对观察药物对细胞代谢的作用是独特的。
To continuously observe the effects of drugs on cell metabolism, paclitaxel-containing perfusate on human oral squamous cell carcinoma KB cells and human colon cancer HCT 8 cells under perfusion conditions were 31P cell spectrometry determination from the two cells in the unmedicated , Administration and after stopping the perfusion conditions obtained metabolic map found that paclitaxel so that KB cells and HCT 8 cells ATP metabolism increased, but the KB cells increased ATP more obvious; the same dose of Vincristine KB cell metabolism in ATP peak changes are not obvious, speculated that may be related to their different mechanisms of action. The results show that perfusion cell 31P spectrometry test for the observation of drugs on cell metabolism is unique.