目的 对比人工培育虫草菌丝和天然冬虫夏草制剂抗衰老、调节免疫等药效作用,以开发冬虫夏草药源。方法 将小鼠分为正常对照组、衰老模型组、天然虫草组、人工虫草组和虫草培养液组,进行抗脂质过氧化、调节免疫等实验。用D-半乳糖造模,用药28d测定全血丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶活性及细胞免疫指标白细胞介素-1、6、8含量。 结果 人工培育虫草菌丝和天然虫草制剂均能明显降低丙二醛含量,升高超氧化物歧化酶、谷胱甘肽过氧化物酶活性,提高白细胞介素-1、6、8含量。结论 人工虫草菌丝与天然虫草均有相似抗衰老作用,故在制剂中可用人工培育的虫草菌丝代替天然虫草。 Objective To compare the anti-aging and immune-regulating effects of artificially cultivated Cordyceps mycelium and natural Cordyceps sinensis preparations to develop the source of Cordyceps sinensis. Methods The mice were divided into normal control group, aging model group, natural Cordyceps group, artificial Cordyceps group and Cordyceps militaris group. Anti-lipid peroxidation and immune regulation were performed. The levels of malondialdehyde, superoxide dismutase, glutathione peroxidase and interleukin-1, 6, 8 in whole blood were measured with D-galactose and 28 days after drug administration. Results Both the artificially cultivated Cordyceps mycelium and the natural Cordyceps sinensis could significantly reduce the content of malondialdehyde, increase the activity of superoxide dismutase and glutathione peroxidase, and increase the content of interleukin-1,6,8. Conclusion Artificial Cordyceps mycelium and natural Cordyceps all have similar anti-aging effects. Therefore, artificial Cordyceps mycelia can be used instead of the natural Cordyceps in the formulation.