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目的:明确养心通脉方主要成分与次要成分之间是否存在着交互作用。方法:从养心通脉方原方中综合提取出人参皂苷、丹参酮ⅡA、人参多糖、总生物碱、总挥发油5个主要成分部位及复方多糖、丹参菲醌2个次要成分部位。根据L4(23)正交设计组成4个处方:①处方1:基础因子+复方多糖、丹参菲醌;②处方2:基础因子;③处方3:基础因子+复方多糖;④处方4:基础因子+丹参菲醌;另设空白对照组,给予双蒸水。观察各成处方组对心肌缺血大鼠模型心电图J点位移及血液流变学指标的影响。结果:造模后第15 s、30 s、120 s、300 s、600 s,4个处方组心电图J点位移变化与空白组比较,差异均具有显著性(均P<0.05),而以处方2组的最明显;4个处方组减轻血液流学变全血切变率的程度较之空白组均具有显著性意义(均<0.01-0.05),其中处方2组改善最为明显。结论:养心通脉方5个主要成分与2个次要成分之间存在着交互作用,而由人参皂苷、丹参酮ⅡA、总生物碱、人参多糖、总挥发油5种成分所组的处方2(即基础因子方),为养心通脉方的最佳有效成分部位方。
OBJECTIVE: To determine whether there is interaction between the main components of Yangxin Tongmai and minor components. METHODS: The five main components of ginsenoside, tanshinone IIA, ginseng polysaccharide, total alkaloid and total volatile oil were extracted from the original formula of Yangxin Tongmai Fang, and compound polysaccharides and two minor components of Salvia miltiorrhiza Bunge. According to L4 (23) orthogonal design composed of four prescriptions: 1 prescription 1: basic factor + compound polysaccharides, Salvia miltiorrhiza; 2 prescription 2: basic factor; 3 prescription 3: basic factor + compound polysaccharide; 4 prescription 4: basic factor + Radix Salviae Miltiorrhizae; another blank control group was given double distilled water. Observe the effect of each prescription group on electrocardiographic J-point displacement and hemorheology in a rat model of myocardial ischemia. RESULTS: Compared with the blank group, the J-point displacement changes of the 4 groups after 15 s, 30 s, 120 s, 300 s, and 600 s were all significant (all P<0.05). The two groups were the most obvious; the four prescription groups had significant differences in reducing the hemorheological blood transfusion rate compared with the blank group (all <0.01-0.05), and the prescription group 2 group had the most obvious improvement. Conclusion: There are interactions between the five main components of Yangxin Tongmai Formula and two minor components, and Formulation 2 consists of five components: ginsenoside, tanshinone IIA, total alkaloid, ginseng polysaccharide, and total volatile oil. That is, the basic factor is the side of the best active ingredient of Yangxin Tongmai.