近年来有些文章推荐用MNT(微核试验)作为化学诱变物体内试验的简单而实用的细胞遗传学筛选方法。为了进一步评价,作者设计了一系列实验,以便肯定此方法。本实验用小鼠以阿糖胞苷进行MNT和染色体分析比较。实验用30g的雄性小鼠,分为6组,每组8只(每种方法用4只)。实验组间隔24小时腹腔注射阿糖胞苷2次,剂量为0.1、2、10、100和400mg/kg。另一组不注射阿糖胞苷作为对照。第2次注射后24小时将小鼠处死取骨髓,按Schmid法做MNT,按Kilian法进行染色体分析。MNT对多染性和正染色红细胞的微 In recent years, some articles recommend using MNT (Micronucleus Test) as a simple and practical method for screening cytogenetics for in vivo testing of chemical mutagens. For further evaluation, the author designed a series of experiments in order to confirm this method. In this experiment, mice were compared with cytarabine for MNT and chromosome analysis. Experimental 30g male mice were divided into 6 groups of 8 (4 for each method). The experimental group was injected intraperitoneally twice a day with cytarabine at doses of 0.1, 2, 10, 100 and 400 mg / kg 24 hours apart. The other group did not receive cytarabine as a control. Twenty-four hours after the second injection, mice were sacrificed to take bone marrow, and MNT was performed according to Schmid’s method. Chromosome analysis was performed according to the Kilian method. MNT of multiple-staining and positive staining erythrocytes