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根据人乳头瘤病毒ＨＰＶ１６，ＨＰＶ１８的核苷酸序列，设计扩增其Ｅ７基因的特定引物，并使引物的５′端引入ＥｃｏＲＩ，ＨｉｎｄⅢ酶切位点，用聚合酶链反应扩增ＨＰＶ１８Ｅ７片段，通过ｐＵＣ１８质粒载体多聚接头的ＥｃｏＲＩ，ＨｉｎｄⅢ双酶切位点与Ｅ７基因片段连接，构建一新的重组体，转化到大肠杆菌ＪＭ１０９，经筛选，快提质粒等鉴定，初步证实获得了ＨＰＶ１８Ｅ７基因的重组体。
Based on the nucleotide sequences of human papillomavirus (HPV16) and human papillomavirus (HPV16), a specific primer was designed to amplify the E7 gene. The 5 ’end of the primer was introduced into EcoRI and HindIII restriction sites, and the HPV18E7 fragment was amplified by polymerase chain reaction The recombinant plasmid pUC18 was cloned into the EcoRI and HindIII restriction sites of the pUC18 plasmid vector and ligated with the E7 gene fragment to construct a new recombinant plasmid. The recombinant plasmid was transformed into E. coli JM109 and identified by screening and rapid plasmid extraction. The HPV18E7 gene Recombination.