核酸分子标记是细胞生物学、微生物学、分子生物学、遗传学、肿瘤学和多种生物测试应用等领域重要的研究方法,也是基因工程主干技术之一.核酸分子标记通常采用灵敏度高的同位素标记,但价格昂贵,检测费时复杂,放射污染等缺点.80年代以来,许多学者致力于非放射性标记物的研究,以便取代同位素应用.本文就非放射性标记方法研究的现状作一综述. Nucleic acid molecular markers are important research methods in the field of cell biology, microbiology, molecular biology, genetics, oncology and a variety of biological testing applications, and are also one of the backbone technologies of genetic engineering.Nucleic acid molecular markers usually use highly sensitive isotopes But expensive, time-consuming and complicated to detect, radioactive pollution, etc. Since the 1980s, many scholars have devoted themselves to the research of non-radioactive labels in order to replace isotope applications.In this paper, the current status of non-radioactive labeling methods is reviewed.