论文部分内容阅读
植物突触融合蛋白(SYP)是一类与植物细胞内囊泡介导转运有关的蛋白。部分SYP基因与植物对生物和非生物胁迫的响应有关。该文利用生物信息学工具分析了木薯(Manihot esculenta)SYP基因及其蛋白结构、核苷酸多态性和系统进化关系,并利用RT-PCR技术检测了木薯不同组织中SYP基因的表达。结果表明,木薯SYP基因及其蛋白结构均具有明显的规律性和家族成员间的保守性;SYP基因的cDNA在基因间以及不同品种间具高度一致性,核苷酸变异以同义替换为主。进化分析表明,植物SYP基因可分为2个亚家族,木薯SYP基因倾向于与蓖麻(Ricinus communis)SYP基因聚在进化树同一分支的末端。半定量RT-PCR分析表明,5个木薯SYP家族成员具有组织特异性。上述研究结果为木薯SYP基因功能研究和功能单核苷酸标记的开发奠定了重要基础。
Plant synaptic fusion protein (SYP) is a class of proteins involved in vesicle-mediated transport in plant cells. Some SYP genes are involved in plant responses to both biotic and abiotic stresses. The bioinformatics tools were used to analyze the SYP gene and its protein structure, nucleotide polymorphism and phylogenetic relationship in cassava (Manihot esculenta). The SYP gene expression in different tissues of Cassava was detected by RT-PCR. The results showed that both the SYP gene and its protein structure of cassava had obvious regularity and conserved among family members. The cDNA of SYP gene was highly consistent among genes and among different varieties, and the nucleotide variation was mainly synonymous . Phylogenetic analysis showed that plant SYP genes were divided into two subfamilies. The cassava SYP gene tended to converge with the SYP gene of Ricinus communis to the end of the same branch of the evolutionary tree. Semi-quantitative RT-PCR analysis showed that five members of the SYP family of cassava are tissue-specific. The above results lay an important foundation for the functional study of cassava SYP gene and the development of functional single nucleotide labeling.