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目的:研究IL-17对原代培养心肌细胞中单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:差速贴壁法分离乳鼠心肌细胞进行原代培养,采用逆转录聚合酶链反应(RT-PCR)检测心肌细胞IL-17R和MCP-1基因表达情况,并采用酶联免疫吸附法(ELISA)检测心肌细胞培养上清中MCP-1蛋白的表达。结果:心肌细胞存在IL-17R的基因表达。IL-17呈浓度依赖式上调心肌细胞MCP-1基因和蛋白的表达水平,与不加刺激因子的对照组相比差异有统计学意义(P<0.05)。心肌细胞MCP-1 mRNA的表达量在IL-17作用4 h时达到高峰,随后开始下降,而IL-17作用下心肌细胞MCP-1蛋白的表达呈时间依赖式升高,与对照组相比有显著性差异(P<0.05)。结论:心肌细胞表达IL-17R。IL-17可上调心肌细胞MCP-1的表达,其作用与IL-17的浓度和作用时间有关。
Objective: To investigate the effect of IL-17 on the expression of monocyte chemoattractant protein-1 (MCP-1) in primary cultured cardiomyocytes. Methods: Primary cultured cardiomyocytes were isolated by differential adherence method. The expression of IL-17R and MCP-1 gene in cardiomyocytes was detected by reverse transcription polymerase chain reaction (RT-PCR) (ELISA) to detect MCP-1 protein expression in cardiomyocyte culture supernatant. Results: The gene expression of IL-17R existed in cardiomyocytes. IL-17 increased MCP-1 gene and protein expression in cardiomyocytes in a concentration-dependent manner, which was significantly different from the control group without stimulation (P <0.05). The expression of MCP-1 mRNA in cardiomyocytes peaked at 4 h after IL-17 treatment and then began to decrease. However, the expression of MCP-1 protein in cardiomyocytes increased in a time-dependent manner compared with the control group There was significant difference (P <0.05). Conclusion: Myocardial cells express IL-17R. IL-17 can up-regulate the expression of MCP-1 in cardiomyocytes, which is related to the concentration and duration of IL-17.