目的:建立UPLC法测定复方守宫散中二苯乙烯苷含量的方法。方法:采用超高效液相色谱法,Acquity BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,乙腈-水(25∶75)为流动相,流速0.25 mL/min,柱温26℃,检测波长320 nm。结果:二苯乙烯苷的进样量在0.1360~1.1350μg(r=0.9996)范围内与其峰面积呈良好的线性关系;平均加样回收率为98.12%,RSD为2.00%。结论:该方法快速、可靠、准确,可作为复方守宫散的质量控制方法。 Objective: To establish a method for the determination of stilbene glycosides in Fufang Shu Gong Gong by UPLC. Methods: The mobile phase was acetonitrile - water (25:75) with a mobile phase of Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm) at a flow rate of 0.25 mL / Wavelength 320 nm. Results: The calibration curve of stilbene glycoside showed a good linear relationship with the peak area in the range of 0.1360-1.1350μg (r = 0.9996). The average recovery was 98.12% and the RSD was 2.00%. Conclusion: The method is rapid, reliable and accurate and can be used as a quality control method for compound prescription.