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目的探讨Toll样受体(Toll-like receptors/TLRs)9基因多态性与子宫腺肌病发病风险的关系。方法采用DNA直接测序法检测50例子宫腺肌病患者(病例组)和50例对照组的TLR9基因rs352139和rs352140位点的多态性,比较各基因型和等位基因的分布情况。结果病例组和对照组中,TLR9基因rs352139位点的TT、CT、CC3种基因型频率分别为38%,40%,22%和48%,32%,20%,两组比较差异无统计学意义(χ2=1.073,P=0.585);T、C等位基因频率分别58%,42%和64%,36%,两组比较差异无统计学意义(χ2=0.757,P=0.384);TLR9基因rs352140位点的TT、CT、CC3种基因型频率分别为20%,40%,40%和18%,38%,44%,两组比较差异无统计学意义(χ2=0.174,P=0.917);T、C等位基因频率分别为40%,60%和37%,63%,两组比较差异无统计学意义(χ2=0.190,P=0.663)。结论 TLR9基因rs352139和rs352140位点多态性与子宫腺肌病的发病风险无关。
Objective To investigate the relationship between Toll-like receptor (TLRs) 9 gene polymorphism and the risk of adenomyosis. Methods The polymorphisms of rs352139 and rs352140 of TLR9 gene in 50 cases of adenomyosis (case group) and 50 cases of control group were detected by DNA direct sequencing. The distribution of each genotype and allele was compared. Results The TT, CT and CC3 genotype frequencies of rs352139 of TLR9 gene were 38%, 40%, 22% and 48%, 32% and 20% respectively in case group and control group. There was no significant difference between the two groups (Χ2 = 1.073, P = 0.585). The frequencies of T and C alleles were 58%, 42%, 64% and 36% respectively. There was no significant difference between the two groups (χ2 = 0.757, The frequencies of TT, CT and CC3 genotypes at rs352140 locus were 20%, 40%, 40%, 18%, 38% and 44% respectively, with no significant difference between the two groups (χ2 = 0.174, P = 0.917 ). The frequencies of T and C alleles were 40%, 60%, 37% and 63% respectively. There was no significant difference between the two groups (χ2 = 0.190, P = 0.663). Conclusion Polymorphisms of rs352139 and rs352140 in TLR9 gene are not associated with the risk of adenomyosis.