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人前列腺特异抗原（ＰＳＡ）基因受雄激素调节，其雄激素应答元件（ＡＲＥ）位于－１７０附近。为确定雄激素对该基因的诱导作用是否受ＡＲＥ上游序列的影响，把ＰＳＡ启动子不同长度的ＤＮＡ片段与无启动子的ＣＡＴ报告基因相连，然后与雄激素受体表达质粒共转染人前列腺细胞ＰＣ－３。结果表明：ＡＲＥ上游（－４０６～－３７１）的一段３６ｂｐ的ＲＦ３６序列可促进雄激素对ＰＳＡ基因的诱导作用。进一步用该ＤＮＡ片段和ＰＣ－３细胞核蛋白进行区带转移测定，发现ＰＣ－３细胞中某些调节蛋白可与该序列结合，暗示该蛋白可能通过与雄激素受体的相互作用影响雄激素对ＰＳＡ启动子的诱导作用。
The human prostate specific antigen (PSA) gene is regulated by androgens, and its androgen response element (ARE) is located near -170. To determine whether the induction of androgens by the gene is influenced by the upstream sequence of ARE, DNA fragments of different lengths of the PSA promoter were ligated to the promoterless CAT reporter gene and then co-transfected with the androgen receptor expression plasmid into the human prostate. Cell PC-3. The results showed that a 36 bp RF36 sequence upstream of ARE (-406--371) could promote the induction of PSA gene by androgen. Further use of this DNA fragment and PC-3 nuclear protein for band transfer assays, found that certain regulatory proteins in PC-3 cells can be combined with the sequence, suggesting that the protein may interact with androgen receptors affect androgen pairs Induction of the PSA promoter.