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目的:比较硫酸铜溶液处理前后8种不同吸附填料的分离效果,选择更适用于分离鳖甲肽类的填料。方法:采用硅胶,聚酰胺树脂,AB-8大孔吸附,凝胶LH10树脂作为填料,并且分别以硫酸铜溶液处理相应填料,以乙醇-水洗脱系统(纯水到纯乙醇,梯度为20%)对鳖甲中肽类进行分离,以双缩脲反应检测各洗脱部位肽类含量及各填料肽类总量回收率为指标进行分离效果评价。结果:硫酸铜溶液未处理AB-8大孔树脂的回收率最高;硫酸铜溶液处理以后硅胶和凝胶LH20的分离效果有了较为明显区别,其中硫酸铜-硅胶的回收率大大提高,硫酸铜-凝胶在60%乙醇部位明显富集了某些肽类;聚酰胺填料则在硫酸铜溶液处理前后,其分离结果无明显差异。结论:AB-8大孔树脂可用于对鳖甲肽类粗分,并有较好分离效果;硫酸铜处理后硅胶和凝胶LH20分离性能有较大改变,起到了一定分离改善作用。
OBJECTIVE: To compare the separation efficiencies of eight different adsorbent packs before and after treatment with copper sulfate solution, and to choose the more suitable packing for the separation of turtle peptides. Methods: Silica gel, polyamide resin, AB-8 macroporous adsorption and gel LH10 resin were used as fillers, and the corresponding fillers were treated with copper sulfate solution respectively. The ethanol-water eluting system %) On the turtle shell in the separation of peptides to biuret reaction to detect the content of each eluted peptide fractions and the total amount of filler recovery as an indicator of the separation effect evaluation. Results: The recovery rate of AB-8 macroporous resin was the highest when copper sulfate solution was not treated. The separation effect of silica gel and gel LH20 was obvious after copper sulfate solution treatment. The recovery rate of copper sulfate-silica gel was greatly improved, - The gel was clearly enriched in some of the peptides at the 60% ethanol fraction; there was no significant difference in the separation results between the polyamide filler and the copper sulfate solution. CONCLUSION: AB-8 macroporous resin can be used for rough fractionation of turtle peptides, and has a good separation effect. After copper sulfate treatment, the separation performance of silica gel and gel LH20 has been greatly changed, playing a role of separation and improvement.