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目的探讨人乳头瘤病毒(HPV)检测在筛查宫颈癌前病变的临床意义。方法收集320例进行宫颈疾病筛查患者宫颈外口、颈管的脱落细胞,采用AutoCytePREP液基细胞学检查及TBC分级诊断标准,HPV检测采用基因芯片技术。所有患者行阴道镜检查并活检,以病理检查结果为金标准。结果 HPV阳性组(146例)液基细胞学检查正常细胞抹片73例,HSIL以上11例,LSIL42例,ASCUS20例;病理检查阳性率38.36%。HPV阴性组(174例)液基细胞学检查正常细胞抹片153例,HSIL0例,LSIL7例,ASCUS14例;病理检查阳性率8.05%。2组患者宫颈活检阳性率比较,差异有统计学意义(P<0.01)。结论 HPV检测在早期发现宫颈癌及癌前病变有重要意义。
Objective To investigate the clinical significance of human papillomavirus (HPV) detection in screening precancerous cervical lesions. Methods 320 cases of exfoliated cervical and cervical canal cells were collected from patients with cervical disease screening. AutoCytePREP liquid-based cytology and TBC grade diagnostic criteria were used. HPV DNA microarray technique was used to detect HPV. All patients colposcopy and biopsy, pathological examination results as the gold standard. Results In the HPV-positive group (146 cases), 73 cases of normal cell smears were detected by liquid-based cytology. There were 11 cases of HSIL above, 42 cases of LSIL and 20 cases of ASCUS. The positive rate of pathological examination was 38.36%. HPV-negative group (174 cases) liquid cytology 153 cases of normal smear, HSIL0 cases, LSIL7 cases, ASCUS14 cases; pathological examination positive rate of 8.05%. The positive rates of cervical biopsy in the two groups were statistically significant (P <0.01). Conclusion HPV detection in early detection of cervical cancer and precancerous lesions of great significance.