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对28例急性早幼粒细胞白血病(APL)进行形态学、免疫学及细胞遗传学(MIC)分型。形态学以异常早幼粒细胞增生为主,胞浆伴有大量颗粒,易见Aner小体、核畸形和核仁,三种亚型主要区别于胞浆颗粒。免疫学CD33呈高表达率(94.6%),CD13表达64.3%,CD2、CD15和CD34分别表达17.9%、7.2%和19.0%,2例M3m型CD34均阳性表达,而HLA-DR-。细胞遗传学26例(92.8%)发现特异性核型结构异常t(15;17)(q22;q21),根据核型2例M3m型得到最后确诊.结果显示APL具有独特的MIC特征。
28 cases of acute promyelocytic leukemia (APL) were morphologically, immunologically, and cytogenetically (MIC) typed. Morphology is characterized by abnormal promyelocytic hyperplasia, cytoplasm with a large number of particles, easy to see Aner bodies, nuclear malformations and nucleoli, the three subtypes are mainly distinguished from cytoplasmic granules. Immunological CD33 showed a high expression rate (94.6%), CD13 expression was 64.3%, CD2, CD15, and CD34 were expressed in 17.9%, 7.2%, and 19.0%, respectively. Two cases of M3m type CD34 were positive. Expression, while HLA-DR-. In cytogenetics (26.8%), specific karyotypic abnormality t(15;17)(q22;q21) was found. According to the karyotype, 2 cases of M3m were finally diagnosed. The results show that APL has unique MIC characteristics.