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目的探讨原发性肝癌(PHC)患者HBV DNA水平与HBV基因分型及乙型肝炎病毒血清标志物(HBV-M)的相关性。方法收集228例原发性肝癌患者(PHC组),同时选取145例慢性乙型肝炎患者(CHB组)作为对照,应用聚合酶链反应(PCR)和酶联免疫吸附试验(ELISA)对两组患者的HBV DNA载量及HBV-M进行检测,HBV DNA阳性者采用基因测序技术对PCR扩增后产物进行基因分型。结果 HBV DNA阳性率PHC组为71.05%(162/228),CHB组为74.48%(108/145),差异无统计学意义。两组HBV基因分型最常见为B型和C型,未检出其他基因型。PHC组HBV基因型以C型为主占57.89%(132/228),其次为B型占11.84%(27/228);CHB组HBV基因型C型占58.62%(85/145),B型占13.79%(20/145)。结论本地区PHC组患者HBV感染率较高,且与HBV感染的病毒复制密切相关,HBV-M以小三阳最为常见,HBV基因型以C型为主,HBV DNA水平稍低于CHB组。HBV-M模式为小三阳的慢性乙型肝炎患者是肝癌的高危人群,更要警惕PHC的发生,应重视该人群的定期体检和复查。
Objective To investigate the relationship between HBV DNA level and HBV genotype and hepatitis B virus (HBV) -M in patients with primary liver cancer (PHC). Methods A total of 228 patients with primary liver cancer (PHC) were enrolled in the study. 145 CHB patients were selected as the control group. PCR and enzyme-linked immunosorbent assay (ELISA) Patients with HBV DNA load and HBV-M were detected, HBV DNA positive by gene sequencing technology to PCR products after genotyping. Results The positive rate of HBV DNA was 71.05% (162/228) in PHC group and 74.48% (108/145) in CHB group, the difference was not statistically significant. The two genotypes of HBV genotypes were the most common type B and C, did not detect other genotypes. The genotypes of HBV in PHC group were 57.89% (132/228) in type C, 11.84% (27/228) in type B, 58.62% (85/145) in type CHB, Accounting for 13.79% (20/145). Conclusions The prevalence of HBV infection in PHC group is higher than that in CHB group, which is closely related to the viral replication in HBV infection. HBV-M is most commonly found in small three positives, genotype C is predominant in HBV genotype, and HBV DNA is slightly lower than CHB group. Chronic hepatitis B patients with positive HBV-M pattern in three positives are high risk groups of liver cancer. More attention should be paid to the occurrence of PHC. Periodic physical examination and review of the population should be emphasized.