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目的 :检测急性淋巴细胞白血病 (AL L)完全缓解后微小残留病 (MRD) ,探讨其临床意义。方法 :应用反转录 -巢式聚合酶链反应 (nest- PCR)检测 AL L 完全缓解后 bcr/abl m RNA。结果 :2 3例 AL L 患者中阳性 5例 ,5例阳性者 2月～ 13月内复发 ;而 MRD阴性的 18例中 2例于缓解后 2月、4月复发 ,余 16例无复发。结论 :AL L治疗完全缓解 (CR)后仍存在 MRD者有较高的复发危险 ,应进行巩固治疗和定期监测 ;MRD阴性可以做为临床疗效观察的客观指标。该方法检测 MRD敏感度高 ,适用于临床。
Objective: To detect the minimal residual disease (MRD) after complete remission of acute lymphoblastic leukemia (AL L) and its clinical significance. Methods :Bcr/abl mRNA was detected by nest-PCR using nest-PCR. RESULTS: Of the 23 AL L patients, 5 cases were positive, 5 cases were positive and recurrence occurred within 2 months to 13 months. Among the 18 cases with MRD negative, 2 cases recurred 2 months and 4 months after remission. The remaining 16 cases had no recurrence. Conclusion: There is still a higher risk of recurrence of MRD after complete remission (CR) of AL L. Consolidation therapy and regular monitoring should be performed. MRD negative can be used as an objective indicator of clinical efficacy. This method detects MRD with high sensitivity and is suitable for clinical use.