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目的 探讨冷冻免疫产生的机制。方法 通过人的肺癌细胞和自身淋巴细胞的体外混合培养,测定各组淋巴细胞转化率(LTR)及其培养上清液中可溶性白细胞介素2受体(SIL2R)和白细胞介素6(IL6)的表达,并用流式细胞仪(FCM)测定各组培养上清液对自身肺癌细胞增殖的影响。结果 ①冷冻组LTR及其培养上清液中SIL2R、IL6的含量明显高于对照组(P<0.01)。②冷冻组培养上清液对自身肺癌细胞的增殖有明显的抑制作用(P<0.01)。结论 ①冷冻肺癌细胞可促进淋巴细胞活化、增殖、分泌细胞因子。②冷冻激活免疫活性细胞,可明显抑制肺癌细胞的增殖。即冷冻能激发机体的抗瘤免疫
Objective To explore the mechanism of freezing immunity. METHODS: Lymphocyte transformation rate (LTR) and soluble interleukin 2 receptor (SIL2R) and interleukin 6 (IL6) in culture supernatants were determined by in vitro mixed culture of human lung cancer cells and autologous lymphocytes. The expression and the flow cytometer (FCM) were used to determine the effect of culture supernatants on proliferation of lung cancer cells. Results 1 The contents of SIL2R and IL6 in the LTR and culture supernatant of the frozen group were significantly higher than those in the control group (P<0.01). 2 The culture supernatant of the frozen group had significant inhibition on the proliferation of lung cancer cells (P<0.01). Conclusion 1 Frozen lung cancer cells can promote lymphocyte activation, proliferation, and secretion of cytokines. 2 Frozen activation of immunocompetent cells can significantly inhibit the proliferation of lung cancer cells. That is, freezing can stimulate the body’s anti-tumor immunity