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目的 :探讨白细胞介素 - 1B- 5 11(interleukin- 1B- 5 11,IL- 1B- 5 11)基因型与慢性牙周炎 (chronicperiodontitis,CP)和冠心病 (coronary heart disease,CHD)易感性的关系。 方法:采用序列特异引物多聚酶链反应(SSP- PCR)法和多聚酶链反应 -限制性片段长度多态性 (PCR- RFL P)法 ,收集中重度 CP12 8例、CHD10 5例、中重度CP和 CHD10 8例和同族健康对照 130例个体的颊粘膜拭子 ,提取 DNA,测定 IL - 1B- 5 11位点的基因型 ,比较各组间基因型检出率的差别。 结果:IL- 1B- 5 11等位基因 2在 CHD中的检出率显著高于健康组 ,而在中重度 CP与健康组、中重度 CP和 CHD与健康组之间的分布差异无统计学意义。结论:IL - 1B- 5 11等位基因 2可能与 CHD遗传易感性相关。
Objective: To investigate the relationship between the genotype of interleukin-1B-5 11 (IL-1B-5 11) and the susceptibility to chronic periodontitis (CP) and coronary heart disease (CHD) Relationship. Methods: 8 cases of moderate-severe CP12, 5 cases of CHD10, moderate-severe CP and moderate-severe CP were collected by polymerase chain reaction-specific polymerase chain reaction (SSP-PCR) and polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) CHD108 cases and healthy controls of 130 cases of individual buccal mucosal swabs, DNA extraction, determination of IL - 1B - 5 11 loci genotypes were compared between the genotype detection rate differences. Results: The detection rate of IL-1B-5 11 allele 2 in CHD was significantly higher than that in healthy group, but no statistical difference was found between moderate and severe CP and healthy group, moderate-severe CP and CHD and healthy group significance. Conclusion: IL - 1B - 5 11 allele 2 may be associated with genetic susceptibility to CHD.