摘要目的为比较脉冲式聚焦超声的3种物理性能(包括致组织液化作用、使通透性增加及温热效应)活体情况下释放肿瘤来源的micro RNA进入血液循环的释放动力学情况。材料与方法所有大鼠实验均获得华盛顿大学动物伦理委员会批准。采用定量逆转录聚合酶链反应阵列分析法识别出大鼠实体瘤细胞系中的Micro RNA生物标志物。大鼠皮下移植这些细胞后,随机分配到3个不同条件的脉冲式聚焦超声组:1通过沸腾法进行组织摧毁造成局部组织液化组,2通过空化效应增加组织通透性组,3轻度(<10℃)加热组 Abstract Objective To compare the release kinetics of tumor-derived microRNA released into the blood circulation in vivo under three kinds of physical properties of pulsed focused ultrasound (including tissue liquefaction, permeability increase and warming effect). Materials and Methods All rat experiments were approved by the University of Washington Animal Ethics Committee. Micro RNA biomarkers in rat solid tumor cell lines were identified using quantitative reverse transcription polymerase chain reaction array analysis. The rats were subcutaneously transplanted with these cells and randomly assigned to three pulsed focused ultrasound groups of different conditions: 1 tissue destruction by boiling method caused local tissue liquefaction group, 2 increased cavitation effect by tissue permeability group, 3 mild (<10 ° C) heating group