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目的通过3种不同方法制备大鼠脊髓损伤致继发性肠道功能障碍的模型,探究各组脊髓损伤致继发性肠道功能障碍模型的表现差别,为脊髓损伤继发肠道功能障碍的相关研究提供稳定的实验动物模型复制方法和实验基础。方法将48只SD大鼠随机分为打击组(A组,n=12)、全横断组(B组,n=12)、钳夹组(C组,n=12)、假手术组(D组,n=12)。于术后1 d、3d、7 d分别对各组进行BBB功能评分;肠肌电慢波测定;血清D-乳酸含量测定;HE染色观察肠组织形态。结果术后1 d、3d、7 d,A、B、C组BBB评分、肌电慢波振幅均低于D组(P<0.05);Chiu肠黏膜评分、血清D-乳酸浓度均显著高于D组(P<0.05)。术后第7天,A组血清D-乳酸浓度和Chiu肠黏膜评分与B、C两组间差异存在显著性(P<0.05)。结论不同方法制备脊髓损伤后,各组均继发肠道功能障碍,其中A组模型复制较为稳定,且死亡率较低,更适用于周期较长的实验研究。
OBJECTIVE: To establish a model of secondary intestinal dysfunction caused by spinal cord injury in rats by three different methods and to explore the differences in the performance of the model of secondary intestinal dysfunction caused by spinal cord injury in each group and to study the effects of spinal cord injury secondary to intestinal dysfunction Related research provides a stable experimental animal model replication method and experimental basis. Methods Forty-eight Sprague-Dawley rats were randomly divided into four groups: A (n = 12), B group (n = 12) Group, n = 12). BBB function score, intestinal EMG wave rate, serum D-lactate content were measured on the 1st, 3rd, and 7th day postoperatively respectively. The morphology of intestinal tissue was observed by HE staining. Results BBB score and EMG wave amplitude in groups A, B and C were significantly lower than those in group D on the 1st, 3rd, 7th day after operation (P <0.05). Chiu intestinal mucosa score and serum D-lactate concentration were significantly higher D group (P <0.05). On the 7th day after operation, there was a significant difference (P <0.05) between serum D-lactate concentration and Chiu intestinal mucosa score and B and C groups. Conclusions After spinal cord injury was prepared by different methods, intestinal dysfunction was secondary to each group. The replication in group A was stable and the mortality rate was lower, which was more suitable for the experimental study with longer period.