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目的:研究孕酮诱导阻断因子(progesterone-induced blocking factor,PIBF)剪接体在正常人和肝细胞性肝癌(hepatocellular carcinoma,HCC)患者中的表达情况。方法:Western bolt检测3例正常肝组织和8例HCC癌及癌旁组织中PIBF剪接体的表达;免疫组化检测48例HCC患者癌及癌周组织中PIBF剪接体的表达;ELISA检测103例正常人及109例HCC患者术前和其中15例术后第5天血清中PIBF剪接体浓度;使用t检验比较其差异。结果:PIBF剪接体在HCC组织中表达低于正常肝组织及癌旁组织;正常人血清PIBF剪接体浓度较HCC患者高;HCC患者术前血清PIBF剪接体浓度较术后低。结论:PIBF剪接体在HCC患者血清及癌组织中低表达,可以为其进一步研究提供新思路。
Objective: To investigate the expression of progesterone-induced blocking factor (PIBF) splice junction in normal human and hepatocellular carcinoma (HCC) patients. Methods: Western blot was used to detect the expression of PIBF splicing in 3 normal liver tissues and 8 HCC tissues and adjacent normal tissues. Immunohistochemistry was used to detect the expression of PIBF splicing in cancerous tissues and cancerous tissues in 48 HCC patients. 103 cases The serum concentrations of PIBF splicing in normal and 109 cases of HCC before operation and 15 days after operation were compared; t-test was used to compare the differences. Results: The expression of PIBF splicing in HCC was lower than that in normal liver and paracancer tissues. The concentration of PIBF splicing in normal human serum was higher than that in HCC. Preoperative serum PIBF spliceosome concentration was lower in HCC patients than that in HCC. Conclusion: The low expression of PIBF spliceosome in serum and cancer tissues of HCC patients may provide new ideas for further research.