海岛棉、陆地棉、中棉的茎尖(5-10mm)培养在改良MS附加IAA和Kt的培养基上,均诱导产生了完整小植株,由此为繁殖植茎尖无性系提供了可能性.改良MS培养基不但适用于棉花二倍体茎尖培养,而且适用于棉花单倍体茎尖培养,这为单倍体的快繁提供了可能性.棉株发育初期的茎尖和腋芽较现蕾后的茎尖和腋芽更易于生根,根、茎、叶分化也好.日温32℃、夜温28℃比试验的其它温度更有利于棉花生根和茎、叶分化. Island tips (5-10 mm) from island cotton, upland cotton, and cotton were induced to produce intact plantlets on modified MS plus IAA and Kt medium, thus providing the possibility of breeding apical meristem clones The modified MS medium is not only suitable for the culture of diploid tip of cotton, but also suitable for the culture of shoot tip of cotton haploid, which provides the possibility of rapid propagation of haploid. The shoot tips and axillary buds were more likely to root after budding, and the roots, stems and leaves were well differentiated.The daily temperature of 32 ℃ and night temperature of 28 ℃ were more conducive to cotton rooting and stem and leaf differentiation than other tested temperature.