【摘 要】
：
建立了高效液相色谱-串联质谱(HPLC-MS/MS)检测猪尿、牛尿和羊尿中苯乙醇胺A残留的方法.尿液经酶解、加入内标物、乙酸乙酯萃取、MCX固相萃取柱净化后,供HPLC-MS/MS检测.采用
【机 构】
：
四川农业大学动物营养研究所,农业部畜产口质量安全监督检验测试中心(成都)
论文部分内容阅读
建立了高效液相色谱-串联质谱(HPLC-MS/MS)检测猪尿、牛尿和羊尿中苯乙醇胺A残留的方法.尿液经酶解、加入内标物、乙酸乙酯萃取、MCX固相萃取柱净化后,供HPLC-MS/MS检测.采用电喷雾离子源正模式,在多反应检测(MRM)模式下分析,苯乙醇胺A定性离子对分别为m/z345/150和345/327,定量离子对为m/z 345/327；苯乙醇胺A内标物定性离子对为m/z348/330,定量离子对为m/z 348/330.苯乙醇胺A含量在0～50.0 μg/L范围内的线性关系良好(R2=0.9990)；检出限为0.03 μg/L,定量限为0.1μg/L.在猪尿、牛尿和羊尿中的回收率分别78.4％ ～ 82.9％,85.7％～93.3％和79.8％ ～ 84.9％；相对标准偏差分别为0.8％～4.2％,1.6％～5.6％和2.9％ ～5.8％.
其他文献
初始疲劳品质取决于材料及其加工工艺,而细节疲劳额定强度DFR代表结构细节材料固有疲劳能力.对不同处理的TC21合金锻态试样DFR疲劳强度进行了研究.结果表明,喷丸强化能有效提
In Mediterranean regions drought is the major factor limiting spring barley and durum wheat grain yields. This study aimed to compare spring barley and durum wh
Inorganic nanoparticles were used to treat cancer cells as well as normal cells. Ca9 Sr (PO4)6(OH)2 nanaparticles were prepared through homogeneous precipitatio
Carbon fiber reinforced concrete (CFRC) is a kind of good electrothermal material. When connected to an external power supply, stable and uniform heat suitable
Simulation models for heterogeneous and simultaneous nucleation and random growth of nuclei were developed in terms of the mechanism of recrystallization and Mo
Food security is a global concern and substantial yield increases in crops are required to feed the growing world population. Mutagenesis is an important tool i
Chlorophyl and anthocyanin contents provide a valuable indicator of the status of a plant’s physiology, but to be more widely utilized it needs to be assessed
The dissolved oxygen (DO) concentration is an important index of water quality. This paper studies the dissolved oxygen recovery of the water and bubble mixture
利用适配体的识别能力和可扩增性,构建了基于微磁珠分离技术的适配体实时定量聚合酶链式反应(PCR)检测方法.通过微磁珠偶联的互补链与适配体序列之间的碱基配对结合,有效除去