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用四甲基偶氮唑盐( MTT) 法,检测了41 例肾移植患者移植前后的致敏淋巴细胞,以探讨致敏淋巴细胞与移植受者的早期排斥的关系。结果表明,急性排斥组(14 例) 受者术前致敏淋巴细胞OD 值平均为0 .56 ±0 .20 ,感染组(5 例)OD 值为0 .56 ±0 .23 ,肾功能稳定组(22 例)OD 值为0 .32 ±0 .05 。肾移植术后以上各组OD 值为0 .46 ±0 .19 、0 .46 ±0 .70 、0 .38 ±0 .08 。急性排斥期应用胸腺嘧啶(ATG) 治疗前后致敏淋巴细胞也有很大的变化。结果提示移植前检测和术后跟踪检测移植受者致敏淋巴细胞状态,有助于预测术后移植肾的早期排斥反应,对ATG 治疗也有一定的指导意义。
Sensitized lymphocytes were detected in 41 renal transplant recipients by MTT assay to investigate the relationship between sensitized lymphocytes and early rejection in transplant recipients. The results showed that acute rejection group (14 patients) preoperative sensitized lymphocytes OD value of 0 on average. 56 ± 0. 20, infection group (5 cases) OD value of 0. 56 ± 0. 23, stable renal function group (22 cases) OD value of 0. 32 ± 0. 05. The OD value of the above groups after renal transplantation was 0. 46 ± 0. 19, 0. 46 ± 0. 70, 0. 38 ± 0. 08. Acute rejection of thymine (ATG) before and after treatment of sensitized lymphocytes also have great changes. The results suggest that pre-transplantation testing and post-tracing testing of sensitized lymphocyte status in transplant recipients may be helpful in predicting the early rejection of postoperative transplant kidneys and may also be of guiding significance for ATG treatment.