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目的探讨缝隙连接蛋白43(Cx43)基因表达与胶质瘤细胞增殖之间的关系,及其在胶质瘤发生发展中的作用,拟为手术后治疗及疗效观察提供客观依据。方法采用原位杂交和免疫组织化学染色方法检测胶质瘤细胞cx43 mRNA、Cx43蛋白和增殖细胞核抗原的表达水平。结果对照脑组织和胶质瘤细胞Cx43 mRNA阳性表达率分别为100%(10/10)和61.70%(29/47),差异具有统计学意义(z=-5.407,p=0.000);低度恶性(WHO Ⅰ～Ⅱ级)胶质瘤细胞Cx43 mRNA阳性表达率为100%(7/7)和93.75%(15/16),高度恶性(WHOⅢ～Ⅳ级)为33.33%(6/18)和16.67%(1/6);Cx43 mRNA阳性表达率与胶质瘤组织病理学分级呈负相关(r=-0.794,P=0.000)。对照脑组织和胶质瘤细胞Cx43蛋白表达水平与Cx43 mRNA基本一致。对照脑组织增殖细胞核抗原表达阴性;不同级别胶质瘤细胞增殖细胞核抗原阳性表达率依次为WHOⅣ级100%(6/6)、WHOⅢ级94.44%(17/18)、WHOⅡ级62.50%(10/16)和WHO 1级42.86%(3/7);增殖细胞核抗原阳性表达率与胶质瘤组织病理学分级呈正相关(r=0.589,P=0.000);WHO Ⅲ～Ⅳ级与WHO Ⅰ～Ⅱ级之间差异具有统计学意义(H=13.239,P=0.000)。Cx43 mRNA与Cx43蛋白表达水平呈正相关(r=0.963,P=0.000),Cx43 mRNA及其蛋白质与增殖细胞核抗原表达水平呈负相关(r=-0.621,p=0.000;r=-0.913,P=0.000)。结论胶质瘤细胞Cx43 mRNA及其蛋白质与增殖细胞核抗原表达水平和肿瘤组织病理学分级呈负相关。提示,Cx43基因表达与肿瘤细胞增殖活性和胶质瘤恶性进展密切相关。
Objective To investigate the relationship between the expression of connexin 43 (Cx43) gene and the proliferation of glioma cells and its role in the development of glioma, and to provide an objective basis for the postoperative treatment and observation of the curative effect. Methods The expression of cx43 mRNA, Cx43 protein and PCNA in glioma cells were detected by in situ hybridization and immunohistochemistry. Results The positive rates of Cx43 mRNA in brain tissue and glioma were 100% (10/10) and 61.70% (29/47), respectively, with statistical significance (z = -5.407, p = 0.000) The positive rates of Cx43 mRNA in malignant (WHO grade Ⅰ ~ Ⅱ) glioma cells were 100% (7/7) and 93.75% (15/16), respectively, and 33.33% (6/18) And 16.67% (1/6), respectively. The positive rate of Cx43 mRNA was negatively correlated with histopathological grade of glioma (r = -0.794, P = 0.000). The levels of Cx43 protein in control brain and glioma cells were almost the same as that of Cx43 mRNA. The positive rates of PCNA in different grades of glioma were 100% (WHO), 94.44% (WHO) and 62.50% (WHO / WHO) 16) and WHO grade 42.86% (3/7) respectively. The positive rate of PCNA expression was positively correlated with the histopathological grade of glioma (r = 0.589, P = 0.000) The difference between the two groups was statistically significant (H = 13.239, P = 0.000). Cx43 mRNA and Cx43 protein expression was positively correlated (r = 0.963, P = 0.000), Cx43 mRNA and protein were negatively correlated with the expression of proliferating cell nuclear antigen (r = -0.621, p = 0.000; r = -0.913, P = 0.000). Conclusion The expression of Cx43 mRNA, protein and proliferating cell nuclear antigen in glioma cells is negatively correlated with tumor histopathological grade. These results suggest that Cx43 gene expression is closely related to tumor cell proliferative activity and malignant progression of glioma.