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报告Gc蛋白的一种分离方法，为免疫制备抗Gc血清制备抗原。硫酸该分级沉淀出含Gc的人血清组份，BlueSepharoseCL－6B亲和色谱除去含Gc硫酸铸分级沉淀的人血清组份中的白蛋白，快速高效液相色谱系统过Alkyl-SuperoseTMHR5／5疏水色谱柱和MonoQHR5／5离子交换柱。聚丙烯酸胺凝胶解离、非解离电泳证明，Gc蛋白得到了彻底纯化。免疫固定显示：纯化出的蛋白确实是Gc蛋白．为1-1型。快速高效液相色谱为蛋白质的纯化提供了新的技术及仪器手段。
A method of isolating Gc protein for the preparation of anti-Gc antigens for the preparation of antigens. Sulfuric acid The fraction containing Gc precipitated human serum component, BlueSepharoseCL-6B affinity chromatography to remove albumin precipitated Gc sulfate fractionated human serum albumin, fast high performance liquid chromatography system Alkyl-SuperoseTM HR5 / 5 hydrophobic chromatography Column and MonoQHR5 / 5 ion exchange column. Polyacrylamide gel dissociation, non-dissociation electrophoresis proved that Gc protein has been completely purified. Immobilization showed that the purified protein was indeed Gc protein. For the 1-1 type. Rapid HPLC provides new techniques and instrumental tools for the purification of proteins.