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用对峙生长法从422株红树内生细菌中筛选到1株对青枯雷尔氏菌(Ralstonia solanacea-rum)、金黄色葡萄球菌(Staphylococcus aureus)、溶藻弧菌(Vibrio alginolyticus)、辣椒疫霉菌(Phytophthora capsici)、香蕉枯萎病菌(Fusarium oxysporum f.sp.cubense)等动植物病原细菌和真菌均具有较强的拮抗作用的海洋细菌CⅢ-1菌株,经形态和生理生化特性及16S rRNA序列分析,鉴定该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens)。用33%硫酸铵从菌株的培养滤液中盐析获得对供试病原菌均具有较强拮抗作用的抗菌活性蛋白,性质测定发现,该抗菌活性蛋白对热不稳定,60°C和100°C下处理10min,其抗菌活性分别下降了62.5%和完全丧失,在pH5.0?10.0范围内均具有抑菌作用,以pH7.0时抗菌活性最强。
A total of 422 mangrove endophytic bacteria were screened by confrontation growth method against Ralstonia solanacearum, Staphylococcus aureus, Vibrio alginolyticus, Phytophthora capsici, Fusarium oxysporum f.sp.cubense and other animal and plant pathogenic bacteria and fungi have strong antagonism of marine bacteria C Ⅲ -1 strain, the morphological and physiological and biochemical characteristics and 16S rRNA Sequence analysis identified the strain as Bacillus amyloliquefaciens. Antibacterial activity protein was obtained by salting out from the culture filtrate of the strain with 33% ammonium sulfate. The antibacterial activity of the antibacterial activity protein was found to be unstable at 60 ° C and 100 ° C After 10min treatment, the antibacterial activity decreased by 62.5% and disappeared completely, respectively. Both of them showed bacteriostatic activity in the range of pH 5.0 to 10.0, and the antibacterial activity was the strongest at pH 7.0.