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目的:研究不同盐碱胁迫对党参种子萌发和幼苗生长的影响,探寻提高其抗性能力的途径。方法:测定不同盐碱胁迫下党参种子的发芽势(G v)、发芽率(G r)、发芽指数(G i)和复萌率,幼苗含水量、叶绿素含量、可溶性蛋白质含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性。结果:对党参种子萌发的抑制影响为Na2CO3>NaCl+Na2CO3>NaCl。随着3种盐碱处理的浓度逐渐增大,党参幼苗中叶绿素含量、幼苗含水量、可溶性蛋白质含量逐渐降低,且与对照组间均有显著性差异(P<0.05),均与盐碱处理浓度呈负相关性。但丙二醛(MDA)含量逐渐增大,与盐碱处理浓度呈正相关性。SOD、POD的活性均随盐碱处理浓度的增大而出现“先上升,后下降”的趋势。结论:不同盐碱胁迫对党参种子的萌发及幼苗的生长都有抑制作用,但抑制程度不同,其中Na2CO3>NaCl+Na2CO3>NaCl。
Objective: To study the effects of different saline-alkali stress on the seed germination and seedling growth of Codonopsis pilosula and explore ways to improve its resistance. Methods: The germination potential (G v), germination rate (G r), germination index (G i) and rate of rejuvenation, seedling water content, chlorophyll content, soluble protein content, (MDA) content, superoxide dismutase (SOD), peroxidase (POD) activity. Results: The effect on the germination of Codonopsis pumila was Na2CO3> NaCl + Na2CO3> NaCl. With the concentration of three kinds of salt-alkali treatment increasing, the chlorophyll content, seedling water content and soluble protein content in Codonopsis pilosula were gradually decreased, and there was significant difference with the control group (P <0.05) Concentration was negatively correlated. However, the content of malondialdehyde (MDA) gradually increased, which was positively correlated with the concentration of salt-alkali treatment. The activities of SOD and POD all showed the trend of “first rising, then falling” as the concentration of saline-alkali treatment increased. CONCLUSION: Different salt - alkali stress inhibited the germination and seedling growth of Codonopsis pilosula, but the degree of inhibition was different, among which Na2CO3> NaCl + Na2CO3> NaCl.