依托ABI公司的ID(Amp FlSTR~®Identifiler~®Plus PCR Amplification Kit)复合荧光检测试剂盒,通过改变实验试剂配比,研究获得最经济、高效的配比体系。一是改变实验体系中的引物、缓冲液、超纯水的配比对实验结果影响不大。二是当模板量减少后,增加PCR的循环数及相应的加热体积,对于法医DNA检测的均衡性及结果不存在明显影响。 Relying on ABI’s ID (Amp FlSTR ~ ®Identifiler ~ ®Plus PCR Amplification Kit) complex fluorescence detection kit, by changing the ratio of experimental reagents, research to obtain the most economical and efficient ratio system. First, change the experimental system of primers, buffer, ultra-pure water ratio on the experimental results have little effect. Second, when the amount of the template is reduced, the number of PCR cycles and the corresponding heating volume are increased. There is no obvious influence on the balance and results of forensic DNA testing.