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目的探讨荧光原位杂交(FISH)技术用于早期难免流产绒毛间期细胞染色体非整倍体异常的临床应用价值。方法采用FISH技术对20例我院住院难免流产病人的流产绒毛进行5条染色体(21、13、18、X和Y)的快速检测。结果被检测的20例样本中,用FISH检测,均获得诊断结果,检测成功率为100%,FISH检测结果中有8例异常,异常率为40%,其中XO是难免流产中胚胎中最多的异常。结论应用FISH技术检测未培养绒毛间期细胞染色体数目异常,具有快速,简便,使用样本量少等优势,具有一定的临床应用价值。
Objective To investigate the clinical value of fluorescence in situ hybridization (FISH) in the detection of abnormal chromosome aneuploidy in early unavoidable miscarriage. Methods Fifteen chromosomes (21, 13, 18, X and Y) were detected by FISH in 20 cases of abortion-prone abortion in our hospital. Results Among the 20 samples tested, the diagnostic results were obtained by FISH. The successful rate was 100%. There were 8 abnormalities in FISH and the abnormal rate was 40%. Among them, XO was the most unavoidable in aborted fetuses abnormal. Conclusion The detection of abnormal chromosome number in uncultured villus cells by FISH has the advantages of fast, simple and small sample size, and has a certain clinical value.