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应用双抗体ＥＬＩＳＡ检测弓形虫循环抗原（ＣＡｇ）阳性者的血液及组织液样本４１例，进行病原学对照检查，结果２９例检获弓形虫，两者的符合率达７０．７％。不同样本中的虫体检出率依次为羊水３／３，乳汁１／１，胚胎组织１／１，脑脊液２１／３０，血清３／６。另外，对２９例疑似中枢神经系统弓形虫病患者，取脑脊液进行不同免疫学指标检测。结果，ＣＡｇ于发病３ｄ即可测出，总阳性数为２６例；ＩＨＡ抗体于发病５ｄ出现阳性，阳性数为４例；ＥＬＩｓＡ一ＩｇＭ及ＩｇＧ抗体在发病７ｄ后逐渐出现阳性，阳性数分别为５例和１例。研究结果证实，ＣＡｇ作为弓形虫早期、急性感染的一个指标，具有重要的诊断学意义。
Twenty-one cases of Toxoplasma circulating antigen (CAg) -positive blood and tissue fluid samples were detected by double-antibody ELISA, and etiological control was performed. Twenty-nine cases were detected with Toxoplasma gondii, the coincidence rate was 70.7%. The detection rate of worms in different samples were as follows: amniotic fluid 3/3, milk 1/1, embryo tissue 1/1, cerebrospinal fluid 21/30 and serum 3/6. In addition, 29 cases of suspected central nervous system toxoplasmosis patients, take different levels of immunological indicators of cerebrospinal fluid testing. Results, CAg in the onset of 3d can be measured, the total number of positive for 26 cases; IHA antibody in the onset of 5d appeared positive, the positive number of 4 cases; ELIsA IgM and IgG antibodies gradually positive after 7d, the positive numbers were 5 cases and 1 case. The results confirmed that CAg, as an indicator of early and acute Toxoplasma gondii infection, has important diagnostic significance.