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本实验将碱性成纤维细胞生长因子（ｂＦＧＦ）应用于大鼠任意皮瓣，观察ｂＦＧＦ对缺血皮瓣的坏死率、皮瓣局部组织中超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ）和一氧化氮（ＮＯ）含量的影响，初步探讨ｂＦＧＦ对皮瓣存活的作用机制。结果显示：ｂＦＧＦ治疗组与肝素盐水组比较：（１）皮瓣坏死率明显降低，皮瓣下肉芽组织层明显增厚，毛细血管和成纤维细胞明显增多；（２）中、远段皮瓣中ＳＯＤ含量在术后２４ｈ、４８ｈ明显升高；（３）中、远段皮瓣中ＭＤＡ含量在２４和４８ｈ明显低于对照组；（４）中、远段皮瓣中ＮＯ含量在术后２４ｈ、４８和９６ｈ均明显低于对照组。结果提示局部应用ｂＦＧＦ能有效降低缺血皮瓣的坏死率，促进局部组织ＳＯＤ水平的升高，降低自由基水平。ｂＦＧＦ有可能在皮瓣缺血的防治方面具有一定的作用。
In this experiment, basic fibroblast growth factor (bFGF) was applied to any skin flap in rats. The necrosis rate of bFGF on ischemic flap, the content of superoxide dismutase (SOD), malondialdehyde (MDA) ) And nitric oxide (NO) content of bFGF on the survival mechanism of the flap. The results showed that: Compared with heparin saline group, the bFGF treatment group showed: (1) the necrosis rate of the flap was significantly reduced, the granulation tissue layer thicken obviously under the flap, and the number of capillaries and fibroblasts increased obviously; (2) (3) The contents of MDA in the middle and distal flap were significantly lower than those in the control group at 24 and 48 hours; (4) The contents of NO in the middle and distal flap were significantly higher after operation 24h, 48 and 96h were significantly lower than the control group. The results suggest that local application of bFGF can effectively reduce the necrosis rate of ischemic flap, promote the local tissue SOD level increased, reduce the level of free radicals. bFGF may have a role in the prevention and treatment of flap ischemia.