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目的探讨血管紧张素Ⅱ1型受体(AT1R)基因多态性与原发性高血压颈动脉粥样硬化之间的关系及其发病机制。方法采用盐析法提取150例原发性高血压患者的白细胞DNA,多聚酶链式反应结合限制性内切酶法检测AT1R基因型;彩色多普勒超声测定颈总动脉、颈内动脉,并与100例健康成年人对照。结果原发性高血压患者的AC基因型频率220%,C等位基因频率187%,均比正常健康组高(P<005);随高血压分级增加,颈动脉内膜中层厚度(IMT)逐渐增厚。结论AT1R基因多态性可能是原发性高血压的遗传因素,高血压是颈动脉粥样硬化的重要易感因素。
Objective To investigate the relationship between angiotensin Ⅱ type 1 receptor (AT1R) gene polymorphism and carotid atherosclerosis in essential hypertension and its pathogenesis. Methods The leukocyte DNA was extracted from 150 patients with essential hypertension by salting-out method. The AT1R genotypes were detected by polymerase chain reaction and restriction endonuclease assay. The common carotid artery and internal carotid artery were measured by color Doppler sonography. 100 healthy adults control. Results The frequency of AC genotype was 220% and the frequency of C allele was 187% in patients with essential hypertension (P <0.05). The carotid artery intima - media thickness (IMT) Gradually thickening. Conclusion AT1R gene polymorphism may be the genetic predisposition to essential hypertension. Hypertension is an important predisposing factor of carotid atherosclerosis.