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目的研究５／６肾切除引起大鼠慢性肾功能衰竭并出现贫血时骨髓细胞红细胞生成素（ＥＰＯ）受体基因表达的变化。方法用二步法切除５／６肾组织诱发大鼠肾功能衰竭并出现贫血，另以盐酸苯肼导致的急性贫血大鼠和正常大鼠为对照。用ＲＴＰＣＲ检测３组大鼠骨髓有核细胞ＥＰＯ受体ｍＲＮＡ表达，并经灰度扫描，与共扩增的β肌动蛋白基因相比，再用ＳｏｕｔｈｅｒｎＢｌｏｔｉｎｇ证实特异性。结果５／６肾切除后５周大鼠出现明显的肾功能衰竭和贫血。正常大鼠、急性贫血大鼠和肾性贫血大鼠骨髓有核细胞ＥＰＯ受体ｍＲＮＡ表达量分别为０２６±００６、０５１±０２４、０１５±００６，急性贫血大鼠ＥＰＯ受体ｍＲＮＡ表达量高于正常大鼠（Ｐ＜００５），而肾性贫血大鼠明显低于正常大鼠（Ｐ＜００１）。结论大鼠在出现慢性肾功能衰竭并出现贫血时，骨髓有核细胞ＥＰＯ受体ｍＲＮＡ表达明显减少，这可能在慢性肾功能衰竭并发贫血过程中起着一定作用。
Objective To investigate the gene expression of erythropoietin (EPO) receptor in bone marrow cells of 5/6 nephrectomy rats with chronic renal failure and anemia. Methods Two-step resection of 5/6 kidney tissue was induced in rats with renal failure and anemia. Another acute anemia induced by phenylhydrazine hydrochloride and normal rats served as controls. RT-PCR was used to detect the expression of EPO receptor mRNA in the bone marrow nucleated cells of three groups, and compared with the co-amplified β-actin gene by gray-scale scanning, Southern blotting was used to confirm the specificity. Results After 5/6 nephrectomy, rats developed obvious renal failure and anemia. The levels of EPO receptor mRNA in bone marrow cells of normal rats, acute anemia rats and renal anemia rats were 026 ± 006, 051 ± 024, 015 ± 006, The expression of EPO receptor mRNA in acute anemia rats was higher than that in normal rats (P <005), while that in renal anemia rats was significantly lower than that in normal rats (P <001). Conclusion In rats with chronic renal failure and anemia, the expression of EPO receptor mRNA in bone marrow nucleated cells decreased significantly, which may play a role in the process of anemia associated with chronic renal failure.