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目的研究柳叶蜡梅水提取浓缩液对SD大鼠母体毒性和胚胎的致畸性。方法交配成功的SD雌鼠64只,随机分为柳叶蜡梅低、中、高剂量组及阴性对照组,采用水提法制备柳叶蜡梅浓缩液,低、中、高剂量组孕鼠分别于妊娠7~16 d连续灌胃给予受试物,每天1次;阴性对照组给予蒸馏水。记录孕鼠孕期一般状况和体重变化,妊娠20 d后剖检孕鼠,检查胎仔数及胎仔形态的变化。结果低、中、高剂量组和对照组大鼠受孕率、孕期总增重、吸收胎鼠数及死胎鼠数比较,差异均无统计学意义(P>0.05)。四组胎鼠肋骨数目、胸骨数目、第五块胸骨呈点状数目及头顶骨骨化不全数目比较,差异均无统计学意义(P>0.05)。结论在本实验剂量和条件下,未见柳叶蜡梅水提取浓缩液对大鼠有母体毒性、胚胎毒性和致畸性。
OBJECTIVE To study the maternal toxicity and embryo teratogenicity of concentrated extract of Ligusticum chinense Mill. Methods Sixty-four SD female mice were randomly divided into three groups: low, medium and high dose of Liu Ye Chuan Mei and negative control group. The rats were given gavage once a day for 7-16 days of gestation, respectively. The negative control group was given distilled water. The general status and body weight of pregnant rats during pregnancy were recorded. The pregnant rats were dissected 20 days after gestation, and the changes of fetal number and fetal morphology were examined. Results There was no significant difference in pregnancy rate, total weight gain during pregnancy, number of fetuses in fetus and dead fetus in low, middle and high dose group and control group (P> 0.05). There was no significant difference in the number of ribs, the number of sternum, the number of the fifth sternum and the number of ossification in the four groups (P> 0.05). Conclusion Under the experimental conditions and dosage, there is no maternal toxicity, embryotoxicity and teratogenicity in rats.