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传统的尾蚴小盖片粘合染制法和尾蚴沾附染制法,其缺点是手续繁杂、费时多、操作难度大,稍不小心,尾蚴断裂多,且必须用70％酒精洗去苦味酸后方可染色。我们采用尾蚴固定法即称为改良的尾蚴固定法,介绍如下: 改良的尾蚴固定又称70％或75％酒精热固定法。此法用70％或75％酒精100～200ml加入50ml的烧杯内,置石棉上以酒精灯加热至75～80℃,将预先在50ml烧杯内逸出的尾蚴迅速倒入加热的酒精内,即行固定。倒入量杯内沉淀后移至70％酒精青霉素瓶内保存待染色。以酸卡红染色为例:将热固定的尾蚴倒入小试管内,吸去酒
The traditional cercaria lamellae adhesion dyeing method and cercariae staining method, the drawback is the complicated procedures, time-consuming, difficult to operate, a little careless, cercaria break more, and must be washed with 70% alcohol picric acid The rear can be dyed. We use cercariae fixation method known as the modified cercaria retention method, described as follows: Modified cercariae fixation, also known as 70% or 75% alcohol heat fixation. This method with 70% or 75% alcohol 100 ~ 200ml 50ml beaker, asbestos on the alcohol lamp heating to 75 ~ 80 ℃, the advance in 50ml beaker escaped cercariae quickly into the heated alcohol, that is, fixed. Pour into the measuring cup after the precipitate was removed to 70% alcohol penicillin bottle to be stored to be dyed. Take acid card red staining as an example: The heat-fixed cercariae into the small test tube, suction to the wine