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77年版《中国药典》测定银黄注射液的含量方法,经实验证明:误差大,重现性差,不能消除成分之间的干扰,在实践中难以执行。以纸层析-紫外分光光度法测定银黄注射液的含量,经回收实验结果良好,黄芩甙回收率平均为95.25%,SD=±1.30。CV=1.36%。绿原酸回收率平均为92.54%,SD=±1.64,CV=1.77%。方法简便合理,易于推广。
The Chinese version of the “Chinese Pharmacopoeia” in the 77th edition determined the content of Yinhuang injection. The experiment proved that the error is large and the reproducibility is poor. The interference between components cannot be eliminated and it is difficult to implement in practice. The content of Yinhuang injection was determined by paper chromatography-ultraviolet spectrophotometry. The results of the recovery experiment were good. The average recovery of Astragalus was 95.25%, SD=±1.30. CV = 1.36%. The average recovery of chlorogenic acid was 92.54%, SD = ± 1.64, and CV = 1.77%. The method is simple and reasonable and easy to promote.