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目的探讨非小细胞肺癌组织中血管内皮生长因子(vascular endothelial growth factor,VEGF)表达和树突细胞浸润的关系及临床意义。方法采用免疫组织化学法检测经外科手术切除的43例非小细胞肺癌组织及15例癌旁组织标本中CD1α、CD83、VEGF、微血管密度(microvessel density,MVD)的表达情况。结果(1)肺癌组织中CD83阳性细胞表达率较癌旁组织减少,VEGF和CD34阳性细胞表达率较癌旁组织增加,差异均有统计学意义(P<0.05);(2)有淋巴结转移组的CD83阳性细胞浸润程度明显高于无淋巴结转移组(P<0.05)。Ⅲ期肺癌VEGF阳性表达率明显高于Ⅰ期和Ⅱ期(P<0.05);(3)肺癌组织VEGF阳性表达组中CD83阳性细胞数量明显减少,而MVD明显增加,与VEGF阴性表达组相比差异均有计学意义(P<0.05)。结论VEGF与肺癌组织中浸润树突细胞(TIDC)的成熟存在负相关,可能参与肺癌组织微血管形成和肿瘤的免疫逃逸。
Objective To investigate the relationship between the expression of vascular endothelial growth factor (VEGF) and dendritic cell infiltration in non-small cell lung cancer (NSCLC) and its clinical significance. Methods The expressions of CD1α, CD83, VEGF and microvessel density (MVD) in 43 specimens of NSCLC and 15 specimens of para-cancerous tissue were detected by immunohistochemistry. Results (1) The positive rates of CD83 positive cells in lung cancer tissues were significantly lower than those in adjacent non-cancerous tissues and the expressions of VEGF and CD34 positive cells were higher than those in para-cancerous tissues (P <0.05). (2) The infiltration of CD83 positive cells was significantly higher than that without lymph node metastasis (P <0.05). The positive expression rate of VEGF in stage Ⅲ lung cancer was significantly higher than that in stage Ⅰ and Ⅱ (P <0.05). (3) The number of CD83 positive cells in lung cancer tissue was significantly decreased, while the MVD was significantly increased, compared with that in VEGF negative group Differences were statistically significant (P <0.05). Conclusions VEGF is negatively correlated with the maturation of infiltrating dendritic cells (TIDC) in lung cancer tissues, which may be involved in the microvessel formation and tumor immune escape in lung cancer.