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要:以12个具有不同HMW GS组成类型的春小麦为材料,在同一氮水平条件下研究不同形态氮肥对春小麦籽粒HMW谷蛋白聚合体的效应。结果表明,春小麦开花后10～15d左右形成可溶性谷蛋白,先出现的是低分子量谷蛋白,接着是HMW谷蛋白聚合体。随籽粒生长谷蛋白积累水平呈上升趋势,花后2 5d达最大值,但品种(品系)间的积累强度、最大值和最终水平均不同;籽粒灌浆过程中先出现较低分子量的HMW谷蛋白聚合体,再形成更高分子量的HMW谷蛋白聚合体。HMW谷蛋白聚合体出现在花后10～2 0d ,具有7+9和2 +12亚基组成的新克旱9、泉2 2 4和东农S5 7于花后2 0d达最大值,其余品种(品系)花后2 5d达最大值,但各品种(品系)积累强度和最终水平存在差异。对于HMW谷蛋白聚合体的积累强度和最终水平的增加尿素具有一定作用,各品种(品系)的遗传特性也有一定影响,但与品种(品系)的HMW GS组成无明显联系。
To study the effect of different nitrogen fertilizers on HMW glutenin aggregates in spring wheat under the same nitrogen level using 12 spring wheat cultivars with different HMW GS compositions. The results showed that soluble gluten was formed about 10-15 days after flowering in spring wheat. The first appeared low molecular weight gluten, followed by HMW glutelin. The accumulation of glutenin increased with the grain growth and reached the maximum at 25 days after flowering, but the accumulation intensity, maximum value and final level were different among cultivars (lines); HMW glutenin Polymer, forming a higher molecular weight HMW gluten polymer. HMW glutenin aggregates appeared at 10 ~ 20 days after anthesis, and the new Khan 9, Quan 2 224 and Dongnong S5 7 with 7 + 9 and 2 + 12 subunits reached the maximum at 20 days after anthesis, and the rest Varieties (lines) reached the maximum on the 25th day after flowering, but there was a difference in the accumulation intensity and final level of all varieties (lines). The accumulation of HMW glutenin aggregates and the final level of urea increased to a certain extent, the genetic characteristics of each variety (lines) also have an impact, but with the varieties (lines) HMW GS composition no significant relationship.