论文部分内容阅读
目的筛选对纳米颗粒物分散效果好、对机体损伤小的纳米颗粒物分散液。方法通过肉眼观察、透射电镜表征及肺损伤指标的测定,观察0.9%生理盐水、新生小牛血清、自制微乳液、1%新生小牛血清白蛋白对纳米颗粒物的分散效果及对机体的毒性作用。结果肉眼观察载带纳米氧化锌的纳米碳颗粒在新生小牛血清中分散均一、稳定时间长,在生理盐水中沉降较快。透射电镜表征结果显示,纳米颗粒在生理盐水中有团聚现象,最大粒径为60nm;在新生小牛血清中分散均匀,粒径为20~40 nm;在微乳液中,纳米碳颗粒外周形成一层包覆,粒径可达100 nm;而在白蛋白分散液中未能观察到颗粒物的粒径大小。纳米颗粒物分散悬液肺毒性比较结果显示,以微乳液、生理盐水制备纳米颗粒物染毒悬液染毒大鼠后,肺泡灌洗液中一氧化氮含量明显升高,分别为(146.41±7.02),(162.34±15.87)μmol/L。以牛血清、白蛋白为分散液制备纳米颗粒物染毒悬液染毒后,NO、总蛋白(TP)含量明显降低,分别为NO(79.05±13.01)(11.45±4.91)μmol/L;TP(0.30±0.03),(0.20±0.01)g/L。纳米颗粒物对大鼠肺组织损伤结果显示,纳米氧化锌组TP(0.50±0.19)gL,NO(144.34±50.88)μmol/L,谷胱甘肽过氧化物酶(GSH-PX)(128.02±68.11)U/(mg prot),明显升高;纳米碳载带氧化锌组TP(0.52±0.14)g/L,NO(154.08±15.41)μmol/L,明显升高。结论新生小牛血清对纳米颗粒物的分散效果较好,对机体产生的损伤相对较小,可准确反映纳米颗粒物的毒性效应。
Objective To screen the dispersion of nano-particles good effect, small damage to the body of nano-particles dispersion. Methods By naked eye observation, transmission electron microscopy and lung injury indicators determination of 0.9% saline, newborn calf serum, homemade microemulsion, 1% of newborn calf serum albumin dispersion effect on the body and toxic effects . Results Macroscopic observation of nano-zinc oxide-loaded nano-carbon particles dispersed in newborn calf serum uniform, stable for a long time, quickly settling in saline. The results of TEM showed that the nanoparticles were agglomerated in physiological saline with a maximum particle size of 60 nm. The nanoparticles were uniformly dispersed in newborn calf serum with the particle size of 20-40 nm. In the microemulsion, Layer coating, the particle size up to 100 nm; and in the albumin dispersion failed to observe the particle size of the particle size. The results of lung toxicity showed that the content of nitric oxide in alveolar lavage fluid increased significantly (146.41 ± 7.02) after being exposed to nanoparticle-containing suspension in microemulsion and saline, , (162.34 ± 15.87) μmol / L. The contents of NO and total protein (TP) were significantly decreased with exposure to the suspension of nano-particles prepared from bovine serum and albumin as NO (79.05 ± 13.01) (11.45 ± 4.91) μmol / L, respectively. TP 0.30 ± 0.03), (0.20 ± 0.01) g / L. The results of nano-particle damage of lung tissue in rats showed that TP (0.50 ± 0.19) gL, NO (144.34 ± 50.88) μmol / L and GSH-PX (128.02 ± 68.11) ) Was significantly higher than that of the control group (P <0.05). The TP (0.52 ± 0.14) g / L and NO (154.08 ± 15.41) μmol / L were significantly increased in the nano- Conclusion Neonatal bovine serum has good dispersion effect on nanoparticles and less damage to the body, which can accurately reflect the toxic effects of nanoparticles.