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建立了一套全场光学相干层析(FFOCT)系统,以实现对生物组织和细胞的高分辨层析成像。该光学系统基于Linnik干涉显微结构。不同于现有FFOCT系统采用光纤束照明方式,采用卤钨灯照明和大数值孔径显微物镜成像,压电陶瓷(PZT)移相,面阵电荷耦合器件(CCD)采集干涉信号,由5步移相算法获取层析图,最终合成三维图像。对整套系统的性能进行了详细的阐述与分析,并通过对洋葱表皮细胞和盆栽树叶的光学层析实验,验证了本系统的可行性和精确度。通过对集成电路内部芯片(英特尔奔腾4,横向分辨率达0.8μm)成像,证明了该系统的分辨率可达到0.7μm×0.5μm(横向×纵向)。提出的系统分辨率高、成本低、结构简单便于调节,为实现高分辨率光学相干层析成像提供了简单易行的方法。
A full-field optical coherence tomography (FFOCT) system was established to enable high-resolution tomography of biological tissues and cells. The optical system is based on the Linnik interference microstructure. Different from the existing FFOCT system using optical fiber bundle illumination method, tungsten halogen lamp illumination and large numerical aperture microscopic objective imaging, PZT phase shift and area array charge-coupled device (CCD) Phase-shifting algorithm to obtain chromatograms, the final synthesis of three-dimensional images. The performance of the whole system was elaborated and analyzed in detail, and the optical chromatography experiments on onion epidermal cells and potted leaves verified the feasibility and accuracy of the system. By imaging the integrated circuit chip (Intel Pentium 4, horizontal resolution 0.8μm), the resolution of this system is proved to be 0.7μm × 0.5μm (horizontal × vertical). The proposed system has the advantages of high resolution, low cost and simple structure. It provides a simple and easy method for high resolution optical coherence tomography.