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目的 研究T细胞免疫后正常小鼠的调节性免疫应答。方法 应用体外扩增的卵清白蛋白 (OVA)特异的T细胞克隆免疫BALB c小鼠 ,3 H TdR掺入法分析细胞增殖 ,3 H TdR标记靶细胞检测杀伤T细胞的杀伤效应 ,间接免疫荧光法分析血清中抗T细胞抗体水平。结果 T细胞免疫后能诱导BALB c小鼠产生调节性T细胞的增殖反应、对靶细胞的杀伤效应以及针对于活化的T细胞的体液免疫应答 ,并进一步降低机体对OVA抗原的应答。结论 T细胞免疫能诱导正常机体的调节性免疫应答
Objective To study the regulatory immune response in normal mice after T cell immunization. Methods BALBc mice were immunized with OVA-specific T cell clones in vitro. Cell proliferation was analyzed by 3 H TdR incorporation assay. The killing effect of cytotoxic T lymphocytes was detected by 3H-labeled TdR cells. Indirect immunofluorescence Analysis of serum anti-T cell antibody levels. Results T cell immunization induced the proliferative response of Treg cells, the killing effect on target cells and the humoral immune response against activated T cells in BALB c mice, and further decreased the body’s response to OVA antigen. Conclusion T cell immunity can induce the normal immune response in vivo