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以一种鲍鱼内脏粗多糖(AVP)为实验原料,以其对小鼠降血糖及糖耐量改善效果、抗氧化效果、脏器保护效果为依据,研究其对四氧嘧啶诱导糖尿病小鼠生理功能的影响。结果表明:多糖组能显著控制小鼠消瘦,高、中剂量具极显著效果(P<0.01),给药3周后体重分别为33.96 g和32.95 g;AVP能明显降低糖尿病小鼠血糖值,中剂量(200 mg/kg)降糖效果最好,给药3周后空腹血糖降至15.45 mmol/L,且在一定范围内能改善小鼠糖耐量异常;治疗3周后,多糖组及药物组小鼠血清SOD和GSH-PX活性增加,MDA含量减少,中剂量组SOD、GSH-PX及MDA含量为155.25 U/mL、529.63μmol/L、14.41μmol/L,与模型组相比有极显著差异(P<0.01),揭示了AVP能清除体内过多自由基、提高糖尿病小鼠抗氧化酶活性,促进受四氧嘧啶损害细胞的修复和再生;与模型组相比,多糖组和药物组均可降低糖尿病小鼠脏器指数,中剂量组肝脏、肾脏和脾脏指数分别为5.23、0.94、0.27,具有显著差异(P<0.05)。
A kind of abalone visceral crude polysaccharide (AVP) was used as the experimental material to study its hypoglycemic and impaired glucose tolerance, anti-oxidation and organ protective effects in mice, and its effects on alloxan-induced diabetic mice physiological function Impact. The results showed that: the polysaccharide group can significantly control mice weight loss, high and medium dose with a significant effect (P <0.01), 3 weeks after administration of body weight were 33.96 g and 32.95 g; AVP can significantly reduce the blood glucose level in diabetic mice, In the middle dose (200 mg / kg) hypoglycemic effect best, fasting blood glucose dropped to 15.45 mmol / L after 3 weeks of administration, and within a certain range can improve glucose tolerance in mice; 3 weeks after treatment, polysaccharide group and drug Compared with the model group, the activity of SOD and GSH-PX increased and the content of MDA decreased in the middle dose group, while the content of SOD, GSH-PX and MDA in middle dose group was 155.25 U / mL, 529.63μmol / L, 14.41μmol / L (P <0.01), revealing that AVP can eliminate excess free radicals in vivo, increase the activity of antioxidant enzymes in diabetic mice and promote the repair and regeneration of alloxan-induced cells. Compared with model group, polysaccharide group and drug The indexes of liver, kidney and spleen of middle dose group were 5.23,0.94,0.27 respectively, which were significantly different (P <0.05).