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SARS(严重急性呼吸综合征)是由一种新型的人类冠状病毒引起的。由于SARS冠状病毒3CL蛋白酶在病毒的复制翻译中起关键作用,所以成为抗SARS药物设计的关键靶标之一。该研究中,利用以pET28a为载体的高效原核表达系统,克隆表达出SARS冠状病毒3CL蛋白酶,然后由NTA-Ni2+亲和层析柱对表达的蛋白进行分离纯化。3CL蛋白酶在大肠杆菌表达系统中的成功表达为今后进一步筛选抗SARS病毒药物奠定基础。
SARS (Severe Acute Respiratory Syndrome) is caused by a new type of human coronavirus. Since SARS-CoV 3CL protease plays a key role in viral replication and translation, it has become one of the key targets for anti-SARS drug design. In this study, SARS coronavirus 3CL protease was cloned and expressed using pET28a-efficient prokaryotic expression system. The expressed protein was isolated and purified by NTA-Ni2 + affinity chromatography. The successful expression of 3CL protease in E. coli expression system lays the foundation for further screening of anti-SARS virus drugs in the future.