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本文基于非制冷焦平面探测器设计了一种新型的可应用于生物医学分析的数字热显微镜,该热显微镜可以获得长波红外显微热图像。研究了热显微镜系统噪声等效温差(NETD)和噪声等效辐射率差(NEED)模型。基于噪声等效温差模型,分析了提高系统温度分辨率的可行性方法。提出了一种基于单幅画面的非制冷焦平面探测器的非均匀校正算法,同时采用了直方图均衡化方法来提高图像的质量。基于VisualC++和已经完成的算法完成了整个系统的软件设计。实际物体的图像采集处理结果表明了该数字热显微镜设计的有效性和合理性。利用该热显微镜,可以完成微小物体的热分析,因此它将成为生物医学分析的有力手段,可以加速该领域的发展并具有广泛的应用前景。
Based on uncooled focal plane detector, a novel digital thermo-microscope that can be applied to biomedical analysis is designed. The thermal microscope can obtain long-wave infrared thermography. The noise equivalent temperature difference (NETD) and the noise equivalent emissivity difference (NEED) model of the thermal microscope system were studied. Based on the noise equivalent temperature difference model, the feasibility of increasing the system temperature resolution is analyzed. A non-uniform correction algorithm for uncooled Focal Plane Detector based on single frame is proposed. At the same time, a histogram equalization method is adopted to improve the image quality. Based on Visual C ++ and has completed the algorithm completed the entire system software design. The actual object image processing results show that the digital thermal microscope design is valid and reasonable. The thermal microscope can be used to perform thermal analysis of small objects, so it will become a powerful tool for biomedical analysis, which can accelerate the development in this field and has a wide range of applications.