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目的探讨细粒棘球蚴(Eg)囊液(HCF)在感染急性期对宿主肝细胞MAPK信号通路及细胞周期的影响。方法常规方法培养HepG2细胞,以HCF为刺激物,于不同时间点用Western blot检测p-ERK、增殖细胞核抗原PC-NA、细胞周期蛋白cyclin-D1、A、B1和E的表达水平,并与无FCS组比较。结果 Western blot检测显示,15%、30%HCF组p-ERK分别在换液培养3h和30min高表达(P<0.05),PCNA分别在3h和12h高表达(P<0.05),cyclin-D1在3h高表达(P<0.05),cyclin-A分别在3h和12h高表达(P<0.05),15%HCF组cyclin-E在3h高表达(P<0.05),15%、30%HCF组cyclin-B1在各时间点表达量微弱(P<0.05)。结论 HCF对体外培养的HepG2细胞无毒害,且对其增殖有一定的促进作用。
Objective To investigate the effects of hydatidiform echinococcosis (Eg) cystic fluid (HCF) on the MAPK signal pathway and cell cycle of host hepatocytes in the acute phase of infection. Methods HepG2 cells were cultured by conventional methods. The expressions of p-ERK, proliferating cell nuclear antigen PC-NA, cyclin D1, cyclin D1, A, B1 and E were detected by Western blot at different time points using HCF as stimulus. No FCS group comparison. Results Western blot showed that the expression of p-ERK in 15% and 30% HCF groups was significantly higher at 3 h and 30 min (P <0.05), and PCNA at 3 and 12 h (P <0.05) (P <0.05). Cyclin-E was highly expressed at 3h and 12h (P <0.05) in 15% HCF group, while cyclin-E was highly expressed at 3h in 15% HCF group -B1 expression at each time point was weak (P <0.05). Conclusion HCF is non-toxic to HepG2 cells cultured in vitro and has certain promotion on its proliferation.