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用蛋白酶切技术制备ＭｃＡｂＪＨⅢＦａｂ′片段，回收率约１０％。片段活性相当于完整ＭｃＡｂ的１１０２稀释浓度。用氯胺Ｔ法标记ＭｃＡｂＪＨⅢＦａｂ′，对荷人肝细胞癌ＢＥＬ－７４０２裸鼠体内的放射免疫显像进行了研究。腹腔注射１３１Ｉ－ＭｃＡｂＪＨⅢＦａｂ′后２４～９６ｈ，肿瘤部位有放射性浓聚，以４８～７２ｈ影像最为清晰；而注射１３１Ｉ－ＭｃＡｂＪＨⅢ肿瘤部位也有积聚现象，但本底消除更慢。１３１Ｉ－ＭｃＡｂＪＨⅢＦａｂ′注射后７２ｈ，１３种器官的肿瘤组织与非瘤组织的放射性比值均大于３，肿瘤的定位指数为２．５６。这表明ＭｃＡｂＪＨⅢＦａｂ′在肝细胞癌的诊断和指导治疗方面有更好的应用前景
McAbJHⅢFab ’fragment was prepared by proteolytic cleavage technique with a recovery of about 10%. Fragment activity corresponds to the 1102 dilution of intact McAb. Radioimmunoimaging in human hepatocellular carcinoma BEL-7402 nude mice was studied by using McAb T method to label McAbJHⅢFab ’. Intraperitoneal injection of 131I-McAbJHⅢFab ’after 24 ~ 96h, the tumor site radioactive accumulation to 48 ~ 72h the most clear image; and injection of 131I-McAbJH Ⅲ tumor sites also accumulated, but the background to eliminate more slowly. At 72h after injection of 131I-McAbJHⅢFab ’, the ratio of radioactivity in tumors and non-tumor tissues of 13 organs was more than 3, and the tumor localization index was 2.56. This shows that McAbJHⅢFab ’has a better application prospect in the diagnosis and treatment of hepatocellular carcinoma