论文部分内容阅读
目的:探讨当归补血汤对大鼠局灶性脑缺血再灌注后大脑皮质神经元的治疗作用。方法:将90只雄性SD大鼠随机分为正常对照组、模型组、当归补血汤(高、中、低剂量)组。观察各组大鼠行为学缺陷、从股静脉注入伊文斯蓝溶液观察血-脑屏障通透性变化、用Nissl染色法观察大脑皮质神经元的形态和数量。结果:与模型组相比较,当归补血汤治疗组各剂量均能改善大鼠神经功能(P<0.05)、减少脑组织中伊文斯蓝含量(P<0.05)、增加大脑皮质神经元数量(P<0.05),并以中剂量组效果最好。结论:当归补血汤对缺血再灌注引发的大脑皮质神经元死亡有保护作用,这可能与其降低血-脑屏障通透性有关。
Objective: To investigate the therapeutic effect of Dangguibuxuetang on cerebral cortex neurons after focal cerebral ischemia-reperfusion in rats. Methods: 90 male Sprague-Dawley rats were randomly divided into normal control group, model group and Dangguibuxuetang (high, medium and low dose) groups. The behavioral defects of the rats in each group were observed. The changes of blood-brain barrier permeability were observed by injecting Evans blue solution from femoral vein. The morphology and number of neurons in cerebral cortex were observed by Nissl staining. Results: Compared with model group, each dose of Dangguibuxue Decoction could improve neurological function (P <0.05), reduce Evans blue content (P <0.05) and increase number of neurons in cerebral cortex <0.05), and the best effect in the middle dose group. Conclusion: Dangguibuxuetang can protect cerebral cortex neurons from ischemia-reperfusion-induced neuronal death, which may be related to the decrease of blood-brain barrier permeability.